Reporter

Part:BBa_K4752007:Design

Designed by: Lang Zeng, Chenyu Li, Huafeng Zheng   Group: iGEM23_HNU-China   (2023-10-06)


NCED3-RUBY reporter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1378
    Illegal XbaI site found at 25
    Illegal PstI site found at 1982
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1378
    Illegal PstI site found at 1982
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1378
    Illegal BglII site found at 509
    Illegal BglII site found at 2014
    Illegal BglII site found at 5737
    Illegal BamHI site found at 1794
    Illegal XhoI site found at 178
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1378
    Illegal XbaI site found at 25
    Illegal PstI site found at 1982
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1378
    Illegal XbaI site found at 25
    Illegal PstI site found at 1982
    Illegal NgoMIV site found at 2340
    Illegal NgoMIV site found at 2919
    Illegal NgoMIV site found at 4614
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1641


Design Notes

The existing pDR5-RUBY plasmid was subjected to enzymatic digestion (enzymes used: SmaI , PstI ), and the digested plasmid was ligated to the corresponding NCED3 promoter using homologous recombination enzymes (ClonExpress II One Step Cloning Kit, Vazyme Biotech).

Source

synthesis

References